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Condensed Matter > Soft Condensed Matter
Title: Measuring vesicle loading with holographic microscopy and bulk light scattering
(Submitted on 12 Apr 2022 (v1), last revised 26 Apr 2024 (this version, v2))
Abstract: We report efforts to quantify the loading of cell-sized lipid vesicles using in-line digital holographic microscopy. This method does not require fluorescent reporters, fluorescent tracers, or radioactive tracers. A single-color LED light source takes the place of conventional illumination to generate holograms rather than bright field images. By modelling the vesicle's scattering in a microscope with a Lorenz-Mie light scattering model, and comparing the results to data holograms, we are able to measure the vesicle's refractive index and thus loading. Performing the same comparison for bulk light scattering measurements enables retrieval of vesicle loading for nanoscale vesicles.
Submission history
From: Anna Wang [view email][v1] Tue, 12 Apr 2022 17:52:35 GMT (8353kb,D)
[v2] Fri, 26 Apr 2024 04:00:53 GMT (12084kb,D)
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