Title: Bioregionalization analyses with the bioregion R-package

Abstract: Bioregionalization consists in the identification of spatial units with similar species composition and is a classical approach in the fields of biogeography and macroecology. The recent emergence of global databases, improvements in computational power, and the development of clustering algorithms coming from the network theory have led to several major updates of the bioregionalizations of many taxa. A typical bioregionalization workflow involves five different steps: formatting the input data, computing a (dis)similarity matrix, selecting a clustering algorithm, evaluating the resulting bioregionalization, and mapping and interpreting the bioregions. For most of these steps, there are many options available in the methods and R packages. Here, we present bioregion, a package that includes all the steps of a bioregionalization workflow under a single architecture, with an exhaustive list of the clustering algorithms used in biogeography and macroecology. These algorithms include (non-)hierarchical algorithms as well as community detection algorithms coming from the network theory. Some key methods from the literature, such as Infomap or OSLOM, that were not available in the R language are included in bioregion. By allowing different methods coming from different fields to communicate easily, bioregion will allow a reproducible and complete comparison of the different bioregionalization methods, which is still missing in the literature.