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Physics > Optics
Title: Multichannel-GaAsP-photomultiplier-based fiber bundle ISM-STED microscope
(Submitted on 18 Apr 2024 (v1), last revised 19 Apr 2024 (this version, v2))
Abstract: The usage of a GaAsP (gallium arsenide phosphide) photomultiplier for microscopical imaging allows the evaluation of low-light luminescent objects. We designed a setup for collecting a confocal microscopic image signal, which is divided into 14 equal-sized input channels. The division is achieved with a beamsplitter and two fiber bundles consisting of seven fibers each. Re-imaging the confocal pinhole by such a densely packed fiber bundle permits the utilization of a photon re-localization approach to overcome the optical resolution limit. The center fiber creates a real-time image, while the outer fibers enable a higher-resolution image via an image scanning microscope (ISM) signal calculation. The fiber bundles are enclosed in a fused silica capillary and are drawn out to create one solid fiber bundle. During the drawing process, the fiber bundles are tapered down to an outer diameter size of 400 {\mu}m, with each fiber having a less than 0.3 Airy unit diameter. For the photomultiplier interface, all fibers of both fiber bundles are integrated into a v-groove array, with each fiber representing a detection input, which is followed by projection optics for imaging onto the multichannel detector. The resulting confocal super-resolution microscope is suitable for the application of time-correlated single photon counting (TCSPC) techniques such as fluorescence lifetime imaging (FLIM), time-resolved anisotropy, or F\"orster resonance energy transfer (FRET) imaging.
Submission history
From: Marcus Babin [view email][v1] Thu, 18 Apr 2024 17:52:05 GMT (29310kb,D)
[v2] Fri, 19 Apr 2024 19:14:04 GMT (24319kb,D)
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